CD127 Monoclonal Antibody (A7R34), Alexa Fluor™ 561, eBioscience™, Invitrogen™

Description

• The A7R34 MAb reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor
• IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes
• In the periphery, mature T cells express CD127 at low level
• A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation
• Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127
• CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis
• The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer
• CD127 consists of an alpha chain and a gamma chain
• The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules
• CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells
• The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor
• Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.