7222818

IgM Monoclonal Antibody (SA-DA4), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IgM

Concentration

5 μL/Test

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias

AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH

Gene Symbols

IGHM

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

IGHM

Clone

SA-DA4

Applications

Flow Cytometry

Conjugate

Brilliant Ultraviolet 737

Host Species

Mouse

Target Species

Human

Gene Accession No.

P01871

Gene ID (Entrez)

3507

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM
  • It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
  • Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific
  • This variation can be attributed to both donor variability and the anticoagulant used during blood draw
  • Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody
  • Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.5 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

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7222818

Mouse Monoclonal Antibody...


Antigen:
IgM

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH

Gene Symbols:
IGHM

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
IGHM

Clone:
SA-DA4

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 737

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P01871

Gene ID (Entrez):
3507

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7222821

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Antigen:
CD3

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
__

Gene Alias:
4930549J05Rik; A430104F18Rik; AI504783; antigen CD3D, delta polypeptide (TiT3 complex); antigen CD3E, epsilon polypeptide (TiT3 complex); antigen CD3G, gamma polypeptide; antigen CD3Z, zeta polypeptide; AW552088; Cd247; CD247 antigen; CD247 antigen, zeta subunit; Cd247 molecule; CD3; CD3 antigen delta chain; CD3 antigen delta polypeptide; CD3 antigen gamma chain; CD3 antigen, delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 epsilon chain; CD3 epsilon subunit; CD3 epsilon subunit precursor; CD3 gamma-chain; CD3 glycoprotein; CD3 glycoprotein precursor; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3 protein; CD3 TCR complex; CD3 type I transmembrane glycoprotein; CD3 type I transmembrane glycoprotein precursor; CD3 zeta chain; Cd3d; CD3D antigen delta; CD3D antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3epsilon; CD3-epsilon; Cd3-eta; Cd3g; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; Cd3h; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); Cd3zeta; Cd3-zeta; CD3zeta chain; CD3-zeta/eta; Ctg3; Ctg-3; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T3/TCR complex; T3d; T3e; T3g; T3Z; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 epsilon chain; T-cell receptor CD3 epsilon subunit; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell receptor zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface protein; TcR CD3 delta-chain; TcR CD3 gamma-chain; TCR zeta chain; TCR zeta chain subunit; TCRE; Tcrk; TCRZ; TCRzeta; TiT3 complex; type I transmembrane protein; T-cell surface molecule CD3

Gene Symbols:
Cd247, Cd3d, CD3E, CD3g

Primary or Secondary:
Primary

Content And Storage:
__

Gene:
__

Clone:
UCHT1

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 805

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P04234, P07766, P09693, P20963

Gene ID (Entrez):
915, 916, 917, 919

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7222822

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Antigen:
CD4

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

Gene Symbols:
CD4

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD4

Clone:
RM4-5

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 805

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P06332

Gene ID (Entrez):
12504

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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7222825

Rat Monoclonal Antibody...


Antigen:
CD8a

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

Gene Symbols:
Cd8a, Cd8b1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Cd8a

Clone:
53-6.7

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 805

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P01731, P10300

Gene ID (Entrez):
12525, 12526

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody