Poly-Ubiquitin Antibody (HWA4C4) - K63 Linkage, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Poly-Ubiquitin
Dilution
Western Blot 1:500 - 1:1000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100
Conjugate
Unconjugated
Regulatory Status
RUO
Formulation
PBS with 0.09% Sodium Azide
Gene ID (Entrez)
6233
Isotype
IgG2a
Purification Method
Ion exchange chromatography
Clone
HWA4C4
Classification
Monoclonal
Host Species
Mouse
Target Species
Non-species specific
Gene Alias
CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80
Immunogen
The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.
Primary or Secondary
Primary
Test Specificity
Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
Description
- Poly-Ubiquitin Monoclonal antibody specifically detects Poly-Ubiquitin in Nonspecies specific samples
- It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).
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Antigen: Poly-Ubiquitin
Dilution: Western Blot 1:500 - 1:1000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS with 0.09% Sodium Azide
Gene ID (Entrez): 6233
Isotype: IgG2a
Purification Method: Ion exchange chromatography
Clone: HWA4C4
Classification: Monoclonal
Host Species: Mouse
Target Species: Non-species specific
Gene Alias: CEP80, HEL112, ribosomal protein S27a, RPS27A, S27A, UBA80, UBC, UBCEP1, UBCEP80
Immunogen: The hybridoma secreting the antibody was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif.
Primary or Secondary: Primary
Test Specificity: Antibody specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitin specific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
Antigen: VISTA/B7-H5/PD-1H
Dilution: ELISA, Immunohistochemistry, Block/Neutralize
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS
Gene ID (Entrez): 64115
Isotype: IgG λ
Purification Method: Protein A purified
Clone: MH5A
Classification: Monoclonal
Host Species: Armenian Hamster
Target Species: Mouse
Gene Alias: B7H5, B7-H5, C10orf54, chromosome 10 open reading frame 54, GI24, platelet receptor Gi24, PP2135, SISP1, stress induced secreted protein 1, VSIR
Immunogen: The antibody was generated by immunising Armenian hamsters with mVISTA/B7-H5/PD-1HIg.
Primary or Secondary: Primary
Test Specificity: __
Antigen: B7-1/CD80
Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Block/Neutralize, Functional Assay
Conjugate: Unconjugated
Regulatory Status: RUO
Formulation: PBS
Gene ID (Entrez): 941
Isotype: IgG κ
Purification Method: Protein A purified
Clone: RM80
Classification: Monoclonal
Host Species: Rabbit
Target Species: Mouse
Gene Alias: Activation B7-1 antigen, B7, BB1, CD28LG1, CD28LGB7-1 antigen), CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Primary or Secondary: Primary
Test Specificity: __
