7241447

CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD170 (Siglec F)

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias

CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F

Gene Symbols

Siglecf

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Siglecf

Clone

1RNM44N

Applications

Flow Cytometry

Conjugate

Brilliant Violet 480

Host Species

Rat

Target Species

Mouse

Gene Accession No.

Q920G3

Gene ID (Entrez)

233186

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • This 1RNM44N mAb recognizes mouse CD170, also known as Siglec F
  • Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types
  • The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding
  • Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells
  • Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells
  • This 1RNM44N antibody will recognize a formaldehyde-fixed epitope
  • Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells
  • BV 480 is a dye that emits at 479nm and is intended for use on cytometers equipped with a violet (405nm) laser
  • Excitation: 440nm; Emission: 479nm; Laser: Violet Laser
  • BV is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
  • Powered by Sirigen
  • SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells
  • This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid
  • The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
  • This molecule is expressed by monocytic/myeloid lineage cells
  • SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus
  • It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

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Antigen:
CD170 (Siglec F)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
CD170; CD33 antigen-like 2; CD33L2; mSiglec-F; OB-binding protein 2; OBBP2; OB-BP2; Obesity-binding protein 2; sialic acid binding Ig like lectin 5; sialic acid binding Ig-like lectin 5; sialic acid binding Ig-like lectin F; sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding immunoglobulin-like lectin 5; sialic acid-binding lectin Siglec-F; Siglec; SIGLEC5; siglec-5; Siglecf; Siglec-F

Gene Symbols:
Siglecf

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Siglecf

Clone:
1RNM44N

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 480

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q920G3

Gene ID (Entrez):
233186

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD31 (PECAM-1)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule

Gene Symbols:
Pecam1

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Pecam1

Clone:
390

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 661

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
Q08481

Gene ID (Entrez):
18613

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
Ki-67

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Gene Symbols:
Mki67

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Mki67

Clone:
SolA15

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 615

Host Species:
Rat

Target Species:
Canine, Cynomolgus Monkey, Human, Mouse, Non-human Primate, Rat

Gene Accession No.:
E9PVX6, P46013

Gene ID (Entrez):
100686578, 102135895, 17345, 291234, 4288

Isotype:
IgG2a κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Antigen:
CD11b

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
antigen CD11b (p170); antigen CD11b (p170); macrophage antigen alpha polypeptide; CD11 antigen-like family member B; CD11b; CD11B (p170); CD11b/CD18; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR3; CR-3 alpha chain; CR3A; F730045J24Rik; integrin alpha M; integrin alpha-M; integrin subunit alpha M; integrin, alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; leukocyte integrin alpha-M chain; LOC100351865; Ly-40; MAC1; Mac-1; Mac-1 alpha; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; MAC1A; Mac-1a; macrophage antigen alpha; macrophage antigen alpha polypeptide; MGC117044; MO1A; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; SLEB6; unnamed protein product

Gene Symbols:
ITGAM

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
ITGAM

Clone:
ICRF44

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 615

Host Species:
Mouse

Target Species:
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Rhesus Monkey

Gene Accession No.:
P11215

Gene ID (Entrez):
101866035, 3684, 454069, 714832

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody