CD8a Monoclonal Antibody (53-6.7), Brilliant Violet™ 421, eBioscience™, Invitrogen™

Description

• The 53-6.7 mAb reacts with the mouse CD8a molecule
• CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha)
• A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha
• CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells
• Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5μg per test BV 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
• Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser
• BV is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
• Powered by Sirigen
• Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation
• CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules
• CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I
• CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha
• Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation
• Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells
• Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.