IL17D Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: APRAGRRPAR PRGCADRPEE LLEQLYGRLA AGVLSAFHHT LQLGPREQAR NASCPAGGRP ADRRFRPPTN LRSVSPWAYR ISYDPARYPR YLPEAYCLCR GCLTGLFGEE DVRFRSAPVY MPTVVLRRTP ACAGGRSVYT EAYVTIPVGC TCVPEPEKDA DSINSSIDKQ GAKLLLGPND APAGP
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17D
• Anti-Human IL-17D-specific antibody was purified by affinity chromatography employing an immobilized Human IL-17D matrix
• Sandwich ELISA: To detect Human IL-17D by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-17D (500-P88Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-17D
• Western Blot: To detect Human IL-17D by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-17D is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• The protein encoded by this gene is a cytokine that shares the sequence similarity with IL17
• The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF
• The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent.