TECK Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSKRNVSLL ISANSGL
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TECK (CCL25)
• Anti-Human TECK (CCL25)-specific antibody was purified by affinity chromatography employing an immobilized Human TECK (CCL25) matrix
• Sandwich ELISA: To detect Human TECK (CCL25) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TECK (CCL25) (500-P134Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TECK (CCL15)
• Western Blot: To detect Human TECK (CCL25) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TECK (CCL25) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus
• Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine
• TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors
• TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes
• Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.