CCL23 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23)
• Anti-Human MIP-3 (CCL23)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3 (CCL23) matrix
• Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23)
• Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• This gene is one of several chemokine genes clustered on the q-arm of chromosome 17
• Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes
• The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide
• This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes
• The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line
• In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1
• Alternative splicing results in multiple transcript variants that encode different isoforms.