NTF4 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Human NT-4
• Anti-Human NT-4-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect hNT-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NT-4 (500-P83G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hNT-4
• Western Blot: To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P83GBT-1MG will be provided as 2 x 500 μg This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons
• The expression of this gene is ubiquitous and less influenced by environmental signals
• While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.