M-CSF Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF
• Anti-Human M-CSF-specific antibody was purified by affinity chromatography employing an immobilized Human M-CSF matrix
• Sandwich ELISA: To detect Human M-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human M-CSF
• Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells
• M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization
• M-CSF is present as several bioactive isoforms that differ in potency and stability
• The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle
• Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit
• Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex
• Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture
• M-CSF acts through the CSF receptor 1
• Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.