75-647-7682

FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor™ Plus 647, Invitrogen™

Manufacturer: LIFE TECHNOLOGIES

Select a Size

Pack Size SKU Availability Price
Each of 1 75-647-7682-Each-of-1 In Stock ₹ 42,352.20

75-647-7682 - Each of 1

₹ 42,352.20

In Stock

Quantity

1

Base Price: ₹ 42,352.20

GST (18%): ₹ 7,623.396

Total Price: ₹ 49,975.596

Antigen

FOXP3

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2

Gene Accession No.

Q9BZS1

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG2a κ

Applications

Immunohistochemistry (Paraffin)

Clone

PCH101

Conjugate

Alexa Fluor Plus 647

Gene

Foxp3

Gene Alias

AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

50943

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

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Description

  • FOXP3 Monoclonal antibody specifically detects FOXP3 in Human samples
  • It is validated for Immunohistochemistry (Paraffin) Description: The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined
  • Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice
  • Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells
  • Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population
  • This is subject to donor variability
  • PCH101 crossreacts with rhesus, chimpanzee and cynomolgus
  • Applications Reported: This PCH101 antibody has been reported for use in immunoblotting (WB) and immunohistology on frozen and paraffin-embedded tissue sections
  • Applications Tested: This PCH101 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 20 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment
  • This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

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Antigen:
FOXP3

Classification:
Monoclonal

Concentration:
0.2 mg/mL

Formulation:
PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2

Gene Accession No.:
Q9BZS1

Host Species:
Rat

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG2a κ

Applications:
Immunohistochemistry (Paraffin)

Clone:
PCH101

Conjugate:
Alexa Fluor Plus 647

Gene:
Foxp3

Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
50943

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Target Species:
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