CD11b Monoclonal Antibody (M1/70), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alphaX
• CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer
• CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells
• CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions
• N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer
• Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 780 can be excited with the violet laser line (405 nm) and emits at 780 nm
• We recommend using a 780/60 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
• The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
• CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
• CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
• CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
• The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
• Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
• Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.