TCR beta Monoclonal Antibody (H57-597), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR
• TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells
• Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions
• H57-597 is used as a phenotypic marker for TCR beta expressing T cells
• Applications Reported: This H57-597 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This H57-597 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
• We recommend using a 780/60 bandpass filter
•  Please make sure that your instrument is capable of detecting this fluorochrome
• In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No
• 01-2222-42) as compared to single-color stained cells
• The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response
• TCR recognition of self-peptides has been linked to autoimmune disease
• Mutant self-peptides have been associated with tumors
• Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome
• Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)
• The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5)
• T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP)
• A small number of T cells lack both CD4 and CD8 (double negative, DN)
• Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders
• Gamma-delta T cells are primarily found within the epithelium
• They show less TCR diversity and recognize antigens differently than alpha-beta T cells
• Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.