Classification:
Monoclonal
Dilution:
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL) Immunofluorescence (1-2 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
Gene Alias:
B220/CD45/CD45R/GP180/LCA/LY5/T200
Purification Method:
Protein A/G purification
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
Description:
Mouse monoclonal antibody raised against full length recombinant human CD45RA.
Formulation:
In 10mM PBS (0.05% BSA, 0.05% sodium azide).
Immunogen:
Recombinant protein corresponding to full length of human CD45RA.
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Content And Storage:
Store at 4°C.