TRITC Polyclonal Antibody, Invitrogen™

Description

• We offer a variety of anti-fluorescent dye antibodies that recognize specific fluorophores and, in most cases, quench their fluorescence
• These anti-dye antibodies, including those that recognize the tetramethylrhodamine (TRITC) and Texas Red™ fluorophores, can also serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria, or other biomolecules have been internalized by endocytic or pinocytic processes
• Our anti-tetramethylrhodamine (A6397) and anti-Texas Red (A6399) antibodies have been raised against the tetramethylrhodamine and Texas Red fluorophores, respectively
• These antibodies quench much of the fluorescence of the complementary dyes
• However, due to the related chemical structures, the anti-tetramethylrhodamine antibody crossreacts with the Texas Red fluorophore and the anti-Texas Red antibody cross-reacts with tetramethylrhodamine
• Also, both antibodies tightly bind the Rhodamine Red™ fluorophore
• Thus, either antibody can serve as an effective probe for tetramethylrhodamine, Texas Red, or Rhodamine Red dyes
• We use a sensitive quenching assay to ensure that this antibody is provided at a consistently high titer value
• As supplied, 20 μL of the antibody solution is certified to produce ≥50% of the maximal fluorescence quenching of 1 mL of a 50 nM solution of tetramethylrhodamine, assayed in 100 mM sodium phosphate, pH 8.0
• TRITC Polyclonal antibody specifically detects TRITC in Chemical samples
• It is validated for Immunocytochemistry, Immunohistochemistry, Western Blot.