APC-AAT complexes Monoclonal Antibody (7), Invitrogen™

Description

• ABS 001-07-02 has specificity for a conformation-dependant neoepitope that is expressed in activated protein C upon complex-formation with alpha1-antitrypsin
• No reaction is seen to non-complexed alpha1-antitrypsin and only very little cross reaction to protein C zymogen
• Note that specificity is calcium dependent
• ABS 001-07-02 has been successfully used in ELISA procedures
• ABS 001-07-02 reacts strongly with APC-AAT complexes in ELISA
• It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum
• Note, that the conformational neoepitope expressed in the complex can also be expressed in APC coated directly onto a high-binding microtiter plate
• The epitope specificity of ABS 001-07-02 is not determined
• NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL The neoepitope is conformation-dependent and expressed in activated protein C upon complex-formation with α1-antitrypsin
• Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains
• The 62 kDa proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation
• The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL
• The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which α1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.