ABS218525UL
Methyl-Glutamine, Rabbit anti-All, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS218525UL-Each-of-1 | In Stock | ₹ 16,456.61 |
ABS218525UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Methyl-Glutamine
Classification
Polyclonal
Dilution
Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany ). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).
Host Species
Rabbit
Molecular Weight of Antigen
∽51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μL
Research Discipline
Signaling
Test Specificity
This rabbit polyclonal antibody detects methylglutamine in proteins.
Content And Storage
Stable for 1 year at -20°C from date of receipt.
Applications
Western Blot
Conjugate
Unconjugated
Formulation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Immunogen
KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
All species
Isotype
IgG
Related Products
Description
- Anti-Methyl-Glutamine, Cat
- No
- ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins
- It has been tested for use in Western Blotting
- Glutamine methylation occurs on translation termination factors and ribosomal proteins
- Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization
- Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors
- Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity
- This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor.
Compare Similar Items
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Antigen: Methyl-Glutamine
Classification: Polyclonal
Dilution: Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany ). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).
Host Species: Rabbit
Molecular Weight of Antigen: ∽51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
Quantity: 25 μL
Research Discipline: Signaling
Test Specificity: This rabbit polyclonal antibody detects methylglutamine in proteins.
Content And Storage: Stable for 1 year at -20°C from date of receipt.
Applications: Western Blot
Conjugate: Unconjugated
Formulation: Rabbit polyclonal antiserum with 0.05% sodium azide.
Immunogen: KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Isotype: IgG
Antigen:
Methyl-Glutamine
Classification:
Polyclonal
Dilution:
Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany ). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).
Host Species:
Rabbit
Molecular Weight of Antigen:
∽51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
Quantity:
25 μL
Research Discipline:
Signaling
Test Specificity:
This rabbit polyclonal antibody detects methylglutamine in proteins.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.
Applications:
Western Blot
Conjugate:
Unconjugated
Formulation:
Rabbit polyclonal antiserum with 0.05% sodium azide.
Immunogen:
KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Isotype:
IgG
Antigen: Methyl-Glutamine
Classification: Polyclonal
Dilution: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: This rabbit polyclonal antibody detects methylglutamine in proteins.
Content And Storage: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: Western Blot
Conjugate: Unconjugated
Formulation: __
Immunogen: KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Isotype: IgG
Antigen:
Methyl-Glutamine
Classification:
Polyclonal
Dilution:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Signaling
Test Specificity:
This rabbit polyclonal antibody detects methylglutamine in proteins.
Content And Storage:
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
Western Blot
Conjugate:
Unconjugated
Formulation:
__
Immunogen:
KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Isotype:
IgG
Antigen: phospho-DBC1 (Thr454)
Classification: Polyclonal
Dilution: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μg
Research Discipline: __
Test Specificity: __
Content And Storage: 2-8°C for 12 months
Applications: Inhibition Assays, Western Blot
Conjugate: Unconjugated
Formulation: Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.
Purification Method: Affinity Purified
Regulatory Status: __
Primary or Secondary: Primary
Target Species: Human
Isotype: __
Antigen:
phospho-DBC1 (Thr454)
Classification:
Polyclonal
Dilution:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μg
Research Discipline:
__
Test Specificity:
__
Content And Storage:
2-8°C for 12 months
Applications:
Inhibition Assays, Western Blot
Conjugate:
Unconjugated
Formulation:
Purified rabbit polyclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.
Purification Method:
Affinity Purified
Regulatory Status:
__
Primary or Secondary:
Primary
Target Species:
Human
Isotype:
__
Antigen: mPTGES-1
Classification: Polyclonal
Dilution: __
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 100 μL
Research Discipline: Signaling
Test Specificity: This polyclonal antibody specifically detects mPGES-1 in human.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications: Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Formulation: __
Immunogen: His-tagged full-length recombinant human microsomal prostaglandin E synthase.
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse, Human
Isotype: IgG
Antigen:
mPTGES-1
Classification:
Polyclonal
Dilution:
__
Host Species:
Rabbit
Molecular Weight of Antigen:
__
Quantity:
100 μL
Research Discipline:
Signaling
Test Specificity:
This polyclonal antibody specifically detects mPGES-1 in human.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications:
Western Blot, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Formulation:
__
Immunogen:
His-tagged full-length recombinant human microsomal prostaglandin E synthase.
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Mouse, Human
Isotype:
IgG