BrdU Monoclonal Antibody (MoBU-1), Invitrogen™

Description

• The MoBU-1 clone has a high specificity for BrdU, in addition to no cross-reactivity with EdU
• This clone can also readily detect bromouridine (BrU) incorporated into RNA or BrdUTP incorporated into DNA strand breaks via the TUNEL assay
• Samples labeled with the Alexa Fluor™ conjugates of MoBU-1 can be directly imaged by fluorescence microscopy or high-content imaging
• The unconjugated form and the biotin conjugate of this clone require a secondary antibody or a streptavidin conjugate for detection, respectively
• The MoBU-1 clone is also available for flow cytometry applications
• Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine
• BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication
• Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA
• Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat
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