Syndecan-4 Rat anti-Mouse, Unlabeled, Clone: KY/8.2, BD

Description

• The KY/8.2 mAb reacts with mouse syndecan-4, a cell-surface heparan sulfate proteoglycan
• Although the ligand(s) for this molecule is unknown, syndecans are known to interact with the heparin-binding domain of growth factors, chemokines, and extracellular matrix components
• Accordingly, heparin inhibits the binding of syndecan-4 to the pre-B cell line BC7.12
• Syndecan-4 is localized to focal adhesions and binds protein-kinase Cα
• Thus, it is involved in cytoskeletal organization and signaling
• Syndecan-4 is expressed on stromal cells and B-lymphocyte precursors in bone marrow, in addition to most splenic B cells and a small population of T lymphocytes
• It is highly expressed on activated B lymphocytes, but is downregulated in isotype-switched memory B cells
• The ectodomain of syndecan-4 is shed from cell surfaces constitutively and in response to specific physiological signals, and the resulting soluble fragments are functional
• Immobilized KY/8.2 mAb induced the formation of dendritic processes on syndecan-4-transfected cells and on anti-CD40-activated splenic B cells
• Other methods of activating B cells have not produced the same effect
• This antibody recognizes an epitope in the N-terminus of syndecan-4, regardless of post-translational modifications
• Host Species: Rat Clone: KY/8.2 Isotype: IgG2a κ Species Reactivity: Mouse Immunogen: BCB10 mouse pre-B cell line ELISA, Flow Cytometry