CD73 Rat anti-Mouse, PE, Clone: TY/23, BD

Description

• The TY/23 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 69 kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities
• 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs
• It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues
• CD73 expression appears to be developmentally regulated on leukocytes
• In the bone marrow, it is found on most CD11b+ myeloid cells and very few CD19+ cells of the B-lymphocyte lineage
• It is not found on CD11b+ cells in the periphery nor on marrow-derived GMCSF-stimulated dendritic cells
• Some peripheral B lymphocytes express CD73, with higher levels on isotype-switched B cells
• The few thymocytes which have detectable surface CD73 are of the CD4-CD8- (double negative) and the single-positive populations
• In the peripheral lymphoid organs, significant subpopulations of the CD4+ and CD8+ T lymphocytes express CD73, with variation in the percentages of CD73-bearing T cells observed among inbred mouse strains
• In the thymus and peripheral lymphoid organs, CD73 is found on endothelia and stromal cells
• CD73 has also been detected on bone marrow and thymic epithelial cell lines, kidney glomeruli and proximal-tubule epithelial cells, and liver endothelial cells and hepatocytes
• The TY/23 mAb inhibits the enzymatic activity of CD73
• Although soluble TY/23 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with PMA, but not with Concanavalin-A or plate-bound anti-CD3ε mAb 145-2C11.