Pre-B Cell Receptor Rat anti-Mouse, Biotin, Clone: SL156, BD

Description

• The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked Lambda 5 (λ5) and VpreB proteins
• The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes
• The SL156 antibody reacts with a conformational epitope of the pre-BCR in transfected X63-Ag8.653 cells but not with surrogate light chain, λ5, or VpreB in the absence of IgH
• It detects pre-BCR on pre-B cell lines, but not on pro-B cell lines or IgM-positive splenocytes
• It also detects pre-BCR on the surface of pre-B cells, but not on Ig light chain (IgL)-positive B lymphocytes, from the bone marrow of normal mice
• It has been noted that the cell-surface expression of pre-BCR is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37°C
• After immunization, cell-surface pre-BCR is detected on a subset of splenic IgL+ germinal-center B lymphocytes
• Host Species: Rat Clone: SL156 Isotype: IgG2a κ Species Reactivity: Mouse Immunogen: Purified soluble complex of recombinant μ IgH, λ5, and VpreB Flow Cytometry