CD24 Rat anti-Mouse, Biotin, Clone: M1/69, BD

Description

• The M1/69 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated, glycosyl-phosphatidylinositol-anchored membrane protein expressed on erythrocytes, granulocytes, monocytes, lymphocytes, and neurons
• Hematopoietic stem cells of the embryonic yolk sac and fetal liver express CD24
• Levels of expression of CD24 vary during differentiation of the T and B cell lineages
• Inthe bone marrow, hematopoietic progenitors acquire CD24 expression upon commitment to the B-lymphocyte lineage
• Immature B cells in the bone marrow express low CD24 levels whereas peripheral B lymphocytes express intermediate to high levels of CD24
• The level of CD24 expression has been reported to rise upon activation of splenic B cells with LPS, but not with CD154 (CD40 Ligand)
• The majority of thymocytes express high levels of CD24, while most mature thymic and peripheral T lymphocytes do not express CD24
• In contrast, TCR-bearing thymocytes which emigrate to the spleen are CD24+
• Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24
• CD24 is not expressed by NK cells, as determined by staining with J11d mAb (Cat
• No
• 553146)
• CD24 is involved in the costimulation of CD4+ T cells by B cells, it is a “co-inducer” of in vitro thymocyte maturation, and it is a ligand of CD62P (P-selectin)
• While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24, they show subtle differences in the level of staining of different lymphocyte populations
• When possible, investigators should continue to use the same monoclonal anti-CD24 antibody as used in previous studies
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.