CD80 Rat anti-Mouse, Unlabeled, Clone: 1G10, BD

Description

• The 1G10 antibody reacts with CD80 (B7-1)
• This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell costimulation via interactions with CD28 and CD152 (CTLA-4)
• CD80 is constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig
• Expression of CD80 is greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours
• It has been reported that activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours
• The 1G10 antibody blocks binding of CTLA-4-Ig to CD80, but it does not block stimulation of T cells by natural antigen-presenting cells
• Preliminary evidence has shown that mAb 16-10A1 (Cat
• no
• 553766) blocks binding of 1G10 mAb to CD80, indicating that the two antibodies may recognize overlapping epitopes on the CD80 molecule
• However, the 16-10A1 mAb recognizes an upregulated antigen on UV-irradiated P815 mastocytoma cells which is not detected by the 1G10 mAb; the cause and significance of this differential reactivity of the two anti-CD80 antibodies is unknown.