CD178.1 Mouse anti-Mouse, Biotin, Clone: KAY-10, BD

Description

• The Kay-10 antibody reacts with CD178.1, the Fas Ligand alloantigen (mFasL.1, CD95 Ligand) expressed on activated T lymphocytes of selected strains of mice (eg, C57BL/6, C3H, MRL, NOD, NZB, NZW, and SJL)
• It does not react with similarly activated T blasts from BALB/c, DBA/1, or DBA/2 mice
• It also reacts with Cos cells transfected with mFasL cDNA derived from C57BL/6 and C3H mice, but not with mFasL cDNA from BALB/c or DBA/2 mice
• In addition, Kay-10 mAb efficiently blocks cytotoxic activity of a C3H T-cell line, but not a BALB/c T-cell line
• Phenotypic and genotypic characterizations of mouse Fas Ligand reveal the existence of two alloantigens: mFasL.1, which is recognized by mAbs Kay-10, MFL3 (Cat
• no
• 555291), and MFL4 (Cat
• no
• 555022), and mFasL.2 (recognized by mAbs MFL3 and MFL4)
• Functional studies suggest that mFasL.2 has higher specific activity than mFasL.1
• In the mouse, FasL is expressed on activated T cell lines and in spleen, testis, and eye
• FasL mRNA has been demonstrated at various levels in bone marrow, thymus, spleen, lymph node, lung, small intestine, testis, and uterus
• Moreover, T-cell activators, but not B-cell activators, enhanced the expression of mRNA was restricted to the T-cell lineage among a panel of cell lines from lymphoid tissues
• Fas Ligand is not functional in mice homozygous for the gld (generalized lymphoproliferative disease) mutation; these mice cannot limit the expansion of activated lymphocytes and develop autoimmune disease
• Fas Ligand is a member of the TNF/NGF family which binds to CD95 (Fas), inducing apoptotic cell death
• This Fas/Fas Ligand interaction is believed to participate in T-cell development, the regulation of immune responses, and cell-mediated cytotoxic mechanisms
• There is mounting evidence that Fas Ligand is also pro-inflammatory, mediating neutrophil extravasation and chemotaxis
• Fas Ligand is released from the surface of transfectant cells by metalloproteinases, and the soluble Fas Ligand may block the activities of the membrane-bound molecule.