CD117 Rat anti-Mouse, PE, Clone: ACK45, BD

Description

• The ACK45 monoclonal antibody specifically binds to CD117 (c-Kit), a transmembrane tyrosine-kinase receptor which is encoded by the Kit gene (formerly dominant white spotting, W )
• The c-Kit ligand (also known as steel factor, stem cell factor, and mast-cell growth factor) encoded by the Kitl gene (formerly steel, Sl), is a co-mitogen for hematopoietic stem cells and the myeloerythroid progenitors and a mast-cell differentiation factor
• The mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the hematopoietic system
• In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including the CD90 (Thy-1)[lo] TER-119[-] CD45R/B220[-] CD11b (Mac-1)[-] Ly-6G (Gr-1)[-] CD4[-] CD8[-] Sca-1 (Ly-6A/E)[+] multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and precursors of B and T lymphocytes
• This widespread expression of CD117 in hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages
• Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development of T lymphocytes
• CD117 is also expressed by mast cells
• The expression of CD117 has been included in protocols to isolate hematopoietic progenitors from fetal and adult peripheral blood
• The specificity and functional activity of mAb ACK45 is very similar to that of the published antibody ACK22; it is reported to block the action of c-Kit in vitro and in vivo.