Cyclin B1 Mouse, Unlabeled, Clone: GNS-11, BD

Description

• During the cell cycle, most eukaryotic cells double in mass, replicate their DNA and then distribute identical copies of their genome to progeny cells during mitosis
• An internal biochemical clock ensures that all the necessary events occur in proper sequence and at the appropriate time
• Cell in M phase contain a dominant regulatory factor known as maturation promoting factor (MPF)
• MPF triggers a variety of enzymatic and ultrastructural changes that are necessary for cell division
• In higher eukaryotes, these mitosis-specific alterations include disassembly of the nuclear envelope, packaging of the DNA into chromosomes and assembly of the mitotic spindle
• Purified MPF is a regulator of a protein kinase cascade and is evolutionarily conserved in all eukaryotic cells ranging from yeast to man
• MPF consists predominantly of two polypeptides, cyclin B1 and p34, and contains protein kinase activity itself
• It is the major M-phase-specific histone H1 kinase, but also phosphorylates a variety of other substrates including lamins, nucleolin, RNA polymerase II, retinoblastoma protein, SV40 large T antigen, p53, and the oncogenes c-src and c-abl
• Cyclin B1 migrates at a reduced molecular weight of 62kDa on SDS-PAGE
• Host Species: Mouse Clone: GNS-11 Isotype: IgG2a Species Reactivity: Human Immunogen: Recombinant Human Cyclin B1 Formula Weight [Chemical]: 62kDa Bioimaging, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blotting