p53 Mouse, FITC, Clone: DO-7, BD

Description

• p53 is a 53kDa nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs
• The gene for p53 is the most commonly mutated gene yet identified in human cancers
• Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs
• The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus
• Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle
• However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins
• DO-7 recognizes human wildtype and mutant p53
• It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53
• DO-7 recognizes an epitope between amino acids 1-45 of the known forms of human p53
• Human recombinant p53 protein was used as immunogen
• Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies in normal tissues
• Clone DO-7 was initially characterized by immunoprecipitation, western blot analysis, immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections, and flow cytometric analysis
• Clone 27-35 is an isotype control monoclonal antibody specific for hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl)
• This hapten is not expressed on human cells or human cell lines
• The DO-7 and 27-35 FITC conjugates are matched in F/P ratios
• The optimum F/P ratio was experimentally determined by flow cytometric analysis
• Host Species: Mouse Clone: DO-7, 27-35 Isotype: IgG2b, IgG2b, κ Species Reactivity: Human Flow Cytometry