CD31 Mouse anti-Rat, Unlabeled, Clone: TLD-3A12, BD

Description

• The TLD-3A12 antibody reacts with CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule)
• CD31 is a ∽130kDa integral membrane glycoprotein, a member of the immunoglobulin super family, that mediates homophilic and heterophilic cell-cell adhesion
• CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes
• In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity
• CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat
• In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leukocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis
• The TLD-3A12 mAb partially blocks the proliferation response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen
• This mouse anti-rat CD31 antibody cross-reacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively
• A suspension of Lewis rat microglial cells were used as the source of the immunogen
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.