CD247 Mouse anti-Human, Unlabeled, Clone: 1D4, BD

Description

• The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules
• Ti is the antigen-specific binding component of the TCR
• On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked α - β heterodimer
• A minor fraction express Ti as a γ- δ heterodimer
• The Ti subunits are Ig-like, each containing V and C regions
• Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits
• CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes
• In the mouse, it consists of five chains, γ(gamma), δ (delta), ε , ζ , and η
• The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively
• Clone 1 ζ 3A1 recognizes an epitope in the C-terminus of mouse CD3 ζ
• A synthetic peptide corresponding to the C-terminus of mouse CD3 ζ (BSACDTYDALHMQTLAPR) was used as immunogen
• Hybridomas were derived from Balb/c spleen cells x NS-1.