PMS2 Mouse, Unlabeled, Clone: A16-4, BD

Description

• The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time
• In bacteria the DNA repair process is accomplished by the MutL, MutH, and MutS proteins
• The MutS protein initially recognizes and binds to mismatched DNA
• Following this, MutH, an endonuclease, and MutL form a complex with MutS and carry out an excision repair mechanism
• When bacteria are deficient in one of these enzymes a mutator phenotype arises characterized by genetic instability
• The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals
• In humans the proteins are called MutS homolog2 (MSH2), MutL homolog (MLH1), and PMS2 which is also a homolog of MutL
• After MSH2 and a partner bind to a mismatched DNA duplex, the complex is joined by a heterodimer of MLH1 and PMS2 which together help facilitate the later steps in mismatch repair
• Two other members of this family, MSH3 and MSH6, can also join the MSH2-containing complex to help facilitate repair
• The reduced molecular weight of PMS2 is ∽100kDa
• mAb A16-4 recognizes human and mouse PMS2
• Recombinant human PMS2 (C-terminal half) was used as immunogen
• Host Species: Mouse Clone: A16-4 Isotype: IgG1 κ Species Reactivity: Human Immunogen: Recombinant Human PMS2 Immunofluorescence, Immunoprecipitation, Western Blotting