CD28 Mouse anti-Human, Unlabeled, Clone: CD28.2, BD

Description

• The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T cells, thymocytes and plasma cells
• CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in T cell-B cell interactions
• It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from those stimulated by the TCR complex
• Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to produce IL-2 and increase intracellular Ca2+ concentration
• This finding suggests the existence of functionally distinct subregions on the CD28 molecule
• CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to induce Ca2+ influx in Jurkat T cells
• This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and cynomolgus macaques
• The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes; however, as described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells being CD28 negative.