BDB557879
Lck (pY505) Mouse anti-Human, Alexa Fluor 488, Clone: 4/LCK-Y505, BD
Manufacturer: BD Biosciences
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB557879-Each-of-1 | In Stock | ₹ 29,432.64 |
BDB557879 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,432.64
GST (18%): ₹ 5,297.875
Total Price: ₹ 34,730.515
Antigen
Lck (pY505)
Classification
Monoclonal
Conjugate
Alexa Fluor 488
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications
Flow Cytometry
Clone
4/LCK-Y505
Formulation
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen
Phosphorylated Human Lck Peptide
Quantity
50 Tests
Research Discipline
Cell Biology
Target Species
Human
Isotype
IgG1
Description
- Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs) that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes and NK cells
- Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains
- The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells via their surface MHC class II and class I molecules, respectively
- The catalytic activity of Lck is regulated by both kinases and phosphatases that control the phosphorylation states of two tyrosine residues that have opposing effects
- Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505 (Y505), located near the carboxy terminus
- Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the protein tyrosine phosphatase, CD45
- When Lck is phosphorylated at this site, it assumes a folded tertiary structure which is enzymatically inactive
- When CD45 dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads to conformational changes in the catalytic domain that induce kinase activity
- However, it has been observed that the inhibitory effect of the phosphorylated Y505 can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and Y505 are phosphorylated together in cells activated by hydrogen peroxide
- Activated Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs) of the T cell receptor (TCR) and thus is critical for activation and development of T lymphocytes
- The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a small part of a complex immunoregulatory cascade that involves additional substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins, and specialized membrane microdomains
- The 4/LCK-Y505 monoclonal antibody recognizes the phosphorylated Y505 of the catalytic domain of Lck.The Alexa Fluor™ 488- conjugated format has been evaluated by flow using a human model system
- However, the unconjugated form of this antibody (Mfr
- No.612390) has been shown to react with human, mouse, and rat in western blot
- A phosphorylated peptide corresponding to residues around Tyrosine-505 from human Lck was used as the immunogen
- Host Species: Mouse Clone: 4/LCK-Y505 Isotype: IgG1 Cross Reactivity: Human Flow Cytometry, Intracellular Staining
Compare Similar Items
Show Difference
Antigen: Lck (pY505)
Classification: Monoclonal
Conjugate: Alexa Fluor 488
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications: Flow Cytometry
Clone: 4/LCK-Y505
Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen: Phosphorylated Human Lck Peptide
Quantity: 50 Tests
Research Discipline: Cell Biology
Target Species: Human
Isotype: IgG1
Antigen:
Lck (pY505)
Classification:
Monoclonal
Conjugate:
Alexa Fluor 488
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications:
Flow Cytometry
Clone:
4/LCK-Y505
Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen:
Phosphorylated Human Lck Peptide
Quantity:
50 Tests
Research Discipline:
Cell Biology
Target Species:
Human
Isotype:
IgG1
Antigen: IgG2a κ
Classification: Monoclonal
Conjugate: Alexa Fluor 700
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Content And Storage: Store vials at 2-8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Applications: Flow Cytometry
Clone: G155-178
Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Immunogen: TNP-keyhole limpet hemocyanin
Quantity: 0.1 mg
Research Discipline: Immunology
Target Species: __
Isotype: IgG2a κ
Antigen:
IgG2a κ
Classification:
Monoclonal
Conjugate:
Alexa Fluor 700
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Content And Storage:
Store vials at 2-8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
Applications:
Flow Cytometry
Clone:
G155-178
Formulation:
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Immunogen:
TNP-keyhole limpet hemocyanin
Quantity:
0.1 mg
Research Discipline:
Immunology
Target Species:
__
Isotype:
IgG2a κ
Antigen: Zap-70 (pY319)/Syk (pY352)
Classification: Monoclonal
Conjugate: PE
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications: Flow Cytometry
Clone: 17A/P-ZAP70
Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen: Human phosphorylated ZAP70 Peptide
Quantity: 50 Tests
Research Discipline: Cell Biology
Target Species: Human, Mouse, Rat
Isotype: IgG1
Antigen:
Zap-70 (pY319)/Syk (pY352)
Classification:
Monoclonal
Conjugate:
PE
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications:
Flow Cytometry
Clone:
17A/P-ZAP70
Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen:
Human phosphorylated ZAP70 Peptide
Quantity:
50 Tests
Research Discipline:
Cell Biology
Target Species:
Human, Mouse, Rat
Isotype:
IgG1
Antigen: __
Classification: __
Conjugate: Alexa Fluor 700
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: __
Primary or Secondary: __
Content And Storage: Undiluted at 4°C
Applications: Flow Cytometry
Clone: MOPC-21
Formulation: __
Immunogen: __
Quantity: 0.1 mg
Research Discipline: __
Target Species: Human
Isotype: IgG1 κ
Antigen:
__
Classification:
__
Conjugate:
Alexa Fluor 700
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
__
Primary or Secondary:
__
Content And Storage:
Undiluted at 4°C
Applications:
Flow Cytometry
Clone:
MOPC-21
Formulation:
__
Immunogen:
__
Quantity:
0.1 mg
Research Discipline:
__
Target Species:
Human
Isotype:
IgG1 κ