BDB557908

T Lymphocyte Activation with control Mouse anti-Mouse, APC, PE, PE-Cy7, Clone: 145-2C11, H1.2F3, PC61, BD

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB557908-Each-of-1 In Stock ₹ 63,057.72

BDB557908 - Each of 1

₹ 63,057.72

In Stock

Quantity

1

Base Price: ₹ 63,057.72

GST (18%): ₹ 11,350.39

Total Price: ₹ 74,408.11

Antigen

T Lymphocyte Activation with control

Classification

Monoclonal

Conjugate

APC/PE/PE-Cyanine7

Purification Method

Affinity Purified

Regulatory Status

RUO

Includes

Mouse T Lymphocyte Activation Antibody Cocktail PE-Cy7 CD25, PE CD69, and APC CD3e (100 tests), Mouse T Lymphocyte Subset Isotype Control PE-Cy7 Rat IgG1, γ, PEHamster IgG1, γ and APC Hamster IgG1, κ (100 tests)

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

145-2C11, H1.2F3, PC61

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Mouse

Description

  • The Mouse T Lymphocyte Activation Antibody Cocktail is a three-color reagent designed to identify activated T lymphocytes by direct immunofluorescent staining with flow cytometric analysis
  • This cocktail consists of the following antibody mixture:PE-Cy7 rat anti-mouse CD25 (clone PC61), PE hamster anti-mouse CD69 (clone H1.2F3), and APC hamster anti-mouse CD3e (clone 145-2C11)
  • The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor achain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested
  • CD25 is also found on some developing B cells in the bone marrow, early developing T cells in the thymus, peripheral CD4+ regulatory T (Treg) cells, and dendritic cells
  • The H1.2F3 antibody reacts with CD69 (Very Early Activation antigen)
  • Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells) neutrophils, and macrophages
  • CD69 is expressed also on thymocytes that are undergoing positive selection
  • The 145-2C11 antibody reacts with the 25-kDa echain of the T-cell receptor-associated CD3 complex, which is expressed on thymocytes, mature T lymphocytes, and NK-T cells of all mouse strains tested
  • The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance
  • The Mouse T Lymphocyte Activation Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulins
  • The use of three different fluorochromes for the labeling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample
  • The expression of the three antigens identifies activated T lymphocytes
  • Additional fluorochrome-labeled reagents may be combined with the Mouse T Lymphocyte Activation Antibody Cocktail, and the Mouse T Lymphocyte Activation Isotype Control, to further characterize activated T-cell subpopulations
  • The Mouse T Lymphocyte Activation Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin consisting of the following: PE-Cy™7 rat IgG1, μ (clone A110-1), PE Armenian hamster IgG1, μ (clone G235-2356) and APC Armenian hamster IgG1, κ (clone A19-3).

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Antigen:
T Lymphocyte Activation with control

Classification:
Monoclonal

Conjugate:
APC/PE/PE-Cyanine7

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Includes:
Mouse T Lymphocyte Activation Antibody Cocktail PE-Cy7 CD25, PE CD69, and APC CD3e (100 tests), Mouse T Lymphocyte Subset Isotype Control PE-Cy7 Rat IgG1, γ, PEHamster IgG1, γ and APC Hamster IgG1, κ (100 tests)

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
145-2C11, H1.2F3, PC61

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Mouse

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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
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Store undiluted at 4°C

Applications:
Flow Cytometry

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Regulatory Status:
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Includes:
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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
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Primary or Secondary:
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