Leukemia Inhibitory Factor Mouse anti-Human, PE, Clone: 1F10, BD

Description

• The 1F10 antibody specifically binds to human Leukemia Inhibitory Factor (LIF) also known as Human Interleukin for DA cells (HILDA), Melanoma-derived Lipoprotein Lipase Inhibitor (MLPLI) and Hepatocyte Stimulating Factor III (HSF III)
• LIF is produced by multiple sources including activated T cells and macrophages, myelomonocytic lineages, fibroblasts, liver, heart and melanoma cells
• LIF regulates the differentiation of embryonic stem cells, neural cells, osteoblasts, adipocytes, hepatocytes and kidney epithelial cells
• Other activities include terminal differentiation in leukemic cells and the stimulation of acute-phase protein synthesis in hepatocytes
• Many of its biological functions parallel those of Interleukin-6, Oncostatin M, Ciliary Neurotrophic Factor, Interleukin-11 and Cardiotrophin-1
• In vivo LIF is important in regulating the inflammatory response by tuning the balance of four systems in the body, namely the immune, the haematopoietic, the nervous and the endocrine systems
• The immunogen used to generate the 1F10 hybridoma was recombinant vaccinia virus encoding the human LIF cytokine
• The LIF cytokine had been engineered to get expressed on the membrane of the infected cell
• The attachment to the membrane was obtained with the glycosylphoshosphatidyl anchor targeting DNA sequence from the DAF molecule (CD55)
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature
• Flow Cytometry