Actin Mouse anti-Human, Alexa Fluor 488, Clone: C4/ACTIN, BD

Description

• Changes in cellular morphology, adhesion, and motility occur through the reorganization of the actin cytoskeleton
• This reorganization of actin filaments results from interactions between actin and actin-binding proteins
• Actin is a 42-kDa protein that is known as G-actin in its monomeric form
• Polymerization of G-actin monomers leads to the generation of flexible filaments, 5-9nm in diameter, called F-actin
• F-actin may be organized in linear bundles called stress fibers or in two-dimensional networks
• The latter are highly concentrated beneath the plasma membrane and form the actin cortex
• Regulation of actin cytoskeletal dynamics occurs through actin-binding proteins
• These proteins bind to G- and/or F-actin and regulate various aspects of actin cytoskeletal dynamics, such as polymerization and depolymerization of actin, cross-linking of actin filaments into bundles, interaction of actin-based structures with membranes and other cytoskeletal elements, and locomotion of actin-based structures
• Thus, the actin cytoskeleton is a complex matrix consisting of G- and F-actin along with the multitude of interactions between these actin forms and a variety of different types of actin-binding proteins
• The C4 monoclonal antibody reacts with all known isoforms of actin in vertebrate muscle and non-muscle cells.