IRF-7 Mouse anti-Human, Alexa Fluor 488, Clone: K40-321, BD

Description

• Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses
• IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-α
• IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I)
• Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways
• The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-ε and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-α promoters
• The K40-321 monoclonal antibody recognizes human IRF-7, regardless of phosphorylation status
• Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7
• Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1
• Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected
• We confirmed that mAb K40-321 does not cross-react with TBK1 by Western blot analysis using the purified antibody
• Intracellular Staining