IKKγ Mouse, Unlabeled, Clone: C73-764, BD

Description

• The transcription factor NF-κB, (nuclear factor kappa-B) is controlled by interaction with an inhibitory subunit, IκB, which restricts NF-κB to the cytoplasm
• Following stimulation by various cytokines or other stimuli, IκB becomes degraded and NF-κB is released to the nucleus
• The release of IκB from NF-κB is a critical step in the activation of NF-κB signal pathways
• A group of proteins form an NF-κB regulatory complex, or signalsome
• Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (aka IKK-1 and IKK-2), which phosphorylate critical residues of IκB, thus targeting it for subsequent degradation
• The IKK complex contains similar amounts of IKKα, IKKβ, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ
• IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK) and MEKK1
• IKKγ interacts preferentially with IKKβ and is required for the activation of the IKK complex
• Thus the IKK kinases play an important role in the activation of NF-κB
• IKKγ migrates as a doublet between 50-55kDa in SDS-PAGE
• Immunoprecipitation, Western Blotting