eNOS Mouse, PE, Clone: 3/eNOS/NOS Type III, BD

Description

• Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO)
• NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity
• In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding
• Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner
• The human forms exhibit 52% amino acid identity
• However, they are distinct gene products of about 155 kDa (nNOS) and 140 kDa (eNOS)
• The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC)
• eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization
• The 33/eNOS monoclonal antibody recognizes eNOS, regardless of phosphorylation status
• The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure)
• Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.