PKCα Mouse anti-Human, Alexa Fluor 647, Clone: 3/PKC, BD

Description

• The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion
• At least eleven isozymes have been described
• These proteins are products of multiple genes and alternative splicing
• Conventional PKC (cPKC) subfamily members (α, β, and γ isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V)
• The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca 2 + , phospholipid, diacylglycerol, or phorbol ester
• However, the the C2-like domains of novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) are Ca 2 + -independent
• The atypical PKC (aPKC) subfamily members (ζ, ι, and λ isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters
• They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members
• The C-terminal region of PKC contains the catalytic domain
• The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors
• PKCα regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions
• The 3/PKCα monoclonal antibody recognizes PKCα, regardless of phosphorylation status, and has been reported to crossreact with PKCβ
• Host Species: Mouse Clone: 3 Isotype: IgG2b, κ Species Reactivity: Human Immunogen: Human PKCα aa
• 270-427 Recombinant Protein Intracellular Staining