Stat3 Mouse, PE, Clone: M59-50, BD

Description

• Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors
• Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation
• Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes
• Stat3 is a 92kDa protein that is activated as a DNA-binding protein through cytokines, such as IL-6, and growth factors, such as EGF
• Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression
• It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1
• Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter
• This site is similar to the GAS element that is present in IFN-γ induced genes
• The M59-50 monoclonal antibody recognizes Stat3 (isoform 1) regardless of phosphorylation status
• The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure)
• Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis
• Host Species: Mouse Clone: M59-50 Isotype: IgG1 λ Species Reactivity: Human Immunogen: Human Stat3 Recombinant Protein Intracellular Staining