CD152 Mouse anti-Human, PE, Clone: BNI3, BD

Description

• Reacts with the “cytolytic T lymphocyte-associated antigen”, CTLA-4
• CTLA-4 is transiently expressed on activated CD28+ T cells and binds to CD80 and CD86 present on antigen presenting cells (APC) with high avidity
• This interaction appears to deliver a negative regulatory signal to the T cell
• There are recent reports that indicate that CTLA-4 is also expressed on B cells when cultured with activated T cells, suggesting a possible role of CTLA-4 in the regulation of B-cell response
• Immobilized BNI3.1 enhances T-cell proliferation induced by CD3 and CD28
• Recent studies have showed that CD152 can be expressed by regulatory T (Treg) cells
• It has been found this antibody can stain the intracellular CD152 on the Treg cells after fixation and permeabilization of cells
• Clone BNI3 also cross-reacts with a subset of peripheral blood lymphocytes of baboon, and both rhesus and cynomolgus macaque monkeys, following Concanavalin A (Con A) treatment
• The distribution of BNI3+ cells following activation is similar to that observed with peripheral blood lymphocytes from normal human donors
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.