FoxP3 Mouse anti-Human, Alexa Fluor 647, Clone: 236A/E7, BD

Description

• The 236A/E7 antibody reacts with the human FoxP3 (Forkhead box protein P3) transcription factor, a member of the forkhead or winged helix family of transcription factors
• The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ CD25+ regulatory T cells and represents a specific marker for these cells
• Flow cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+ CD25+ (high) T cells in peripheral blood while less than half of CD4+ CD25+ (intermediate) cell population are FoxP3 positive
• Approximately 5-10% of peripheral CD4+ cells are CD4+ CD25+ T regulatory cells
• T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells
• To support this hypothesis, it has been found that Foxp3 is mutated in scurfy (sf) mice
• Cumulative evidence suggests that the 236A/E7 antibody recognizes epitopes from both isoforms of Exon 2 alternatively-spliced variants and is located in the region between Exon 2 and the Zn/LZ domains (aa 105-235)
• The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed
• The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed
• Host Species: Mouse Clone: 236A/E7 Isotype: Mouse IgG1κ Species Reactivity: Human Immunogen: FoxP3 recombinant protein Intracellular Staining