CD8 Mouse anti-Human, V450, Clone: RPA-T8, BD

Description

• The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α)
• CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily
• CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells
• Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers
• CD8 plays important roles in T cell activation and selection
• The extracellular IgSF domain of CD8 α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen
• The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling
• The RPA-T8 and HIT8a monoclonal antibodies are not cross-blocking
• This clone has been reported to react with a subset of peripheral blood lymphocytes, but not monocytes nor granuloyctes, of baboon and both rhesus and cynomolgus macaque monkey
• In general, a higher frequency of CD8+ and CD4+CD8+ lymphocytes are observed in non-human primates compared to normal human donors
• The antibody is conjugated to BD Horizon V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser Ex max of 406nm and has an Em Max at 450 nm
• Conjugates with BD Horizon V450 can be used in place of Pacific Blue™ conjugates.