ZAP70 (pY319)/Syk (Y352) Mouse anti-Human, PE-Cy7, Clone: 17A/P-ZAP70, BD

Description

• ZAP70 is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation
• ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus
• It appears that both ZAP70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation
• TCR stimulation leads to autophosphorylation of ZAP70 at Tyr-315 amd Tyr-319, and mutation of the Tyr-319 site dramatically impairs TCR signaling
• In addition, TCR-mediated Lck activity leads to phosphorylation of ZAP70 on Tyr-493 in the regulatory loop of the kinase domain leading to upregulation of ZAP70 kinase activity
• The significance of ZAP70 activation in mediating TCR signal transduction has been confirmed by showing that ZAP70 activity is absent in an autosomal recessive form of severe combined immunodeficiency (SCID)
• This is due to mutations affecting the ZAP70 kinase domain which affect the stability of the protein and TCR signaling
• Clone 17A/P-ZAP70 recognizes the phosphorylated form of ZAP70 (Y319)
• It also cross-reacts with SYK (Y352) due to homology of the phosphorylation site with ZAP70 (Y319)
• The PE-conjugated format has been evaluated using human and mouse model systems
• The unconjugated form of the antibody (Cat
• No
• 612574) has also been shown to work in western blot analysis on human, mouse,and rat cells.