Oct3/4A Mouse anti-Human, PE, Clone: O50-808, BD

Description

• Development of a multicellular organism from a single fertilized egg is regulated by the coordinated activity of DNA transcription factors
• Oct3/4, a member of the POU family of transcription factors, functions in pluripotent cells of early embryonic stem (ES) cell lines and embryonal carcinomas (EC)
• The human POU5F1 gene can encode various splice variants, two of which are Oct3/4A and Oct3/4B
• Both isoforms share identical POU DNA-binding and C-terminal domains but differ in their N-terminal domain
• The N-terminal domain of Oct3/4B is inhibitory to the DNA binding domain and therefore cannot stimulate transcription of Oct3/4-dependent genes
• Oct3/4B can be detected in both pluripotent and some differentiated cell types in both the nucleus and cytoplasm, but its function is unclear
• There is not an equivalent to Oct3/4B in mouse
• Oct3/4A is expressed in the nucleus and has been demonstrated to orchestrate the transcription of Oct3/4-dependent genes
• It has been demonstrated that the expression of Oct3/4 isoforms can vary greatly in different cell types, and discrimination of these is crucial for assessing Oct3/4 expression and function
• The O50-808 monoclonal antibody recognizes human Oct3/4 Isoform A and mouse Oct3/4.