CD15 Mouse anti-Human, V500, Clone: HI98 (also known as HIM1), BD

Description

• The HI98 monoclonal antibody specifically reacts with 3-fucosyl-N-acetyllactosamine (3-FAL), a 220 kDa carbohydrate structure, also called X-hapten, SSEA-1, CD15 or Lewis X
• This structure is found on a variety of cell surface glycolipids and glycoproteins
• 3-FAL is expressed on >95% of granulocytes, including neutrophils and eosinophils, and to a varying degree on monocytes, but not on lymphocytes or basophils
• CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis
• This antibody is also suitable for staining formalin-fixed, paraffin-embedded tissue sections without pretreatment
• Since the Abs are recognizing a carbohydrate epitope (3-fucosyl-N-acetyllactosamine) they also should work across species and not only for human
• The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences
• It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500 nm
• BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
• For more information on BD Horizon V500, visit bdbiosciences.com/colors
• When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
• Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g
• CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.