KLRG1 Hamster anti-Mouse, Alexa Fluor 488, Clone: 2F1, BD

Description

• The 2F1 monoclonal antibody specifically binds to KLRG1 ( K iller cell L ectin-like R), which is the mouse homologue of the rat mast cell function-associated antigen (MAFA),on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J)
• Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells
• The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytesor reduced effector functions of activated NK cells
• This molecule is believed to play a common role in the regulation of leukocytes of both the innate and adaptive immune system
• It has been observed that the 2F1 mAb stains the rat basophilic leukemia cell line, RBL-2H3, which is known toexpress MAFA
• The KLRG1 protein is an inhibitory lectin-like type IItransmembrane receptor containing a cytoplasmic motif similar to ITIM ( I mmunoreceptor T yrosine-based I nhibitory M otif);its ligand has not beenidentified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa
• The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected
• Cross-linking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++mobilization and cytotoxic responses (but not IFN-γ production) by CD8+ T cellsand inhibits IFN-γ