BDB561711

CD14 Rat anti-Mouse, PE, Clone: rmC5-3, BD

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB561711-Each-of-1 In Stock ₹ 7,529.28

BDB561711 - Each of 1

₹ 7,529.28

In Stock

Quantity

1

Base Price: ₹ 7,529.28

GST (18%): ₹ 1,355.27

Total Price: ₹ 8,884.55

Antigen

CD14

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

Recombinant Mouse CD14

Quantity

25 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

rmC5-3

Conjugate

PE

Host Species

Rat

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG1 κ

Description

  • The rmC5-3 monoclonal antibody specifically binds to residues 308-322 of the hydrophilic region of mouse CD14
  • CD14 is a 53-55 kDa glycophosphatidylinositol (GPI)-linked glycoprotein belonging to the leucine-rich glycoprotein repeat superfamily of cell-surface proteins
  • It is a receptor for the complex of lipopolysaccharide (LPS or endotoxin, from gram-negative bacteria) with LPS-binding protein (LBP, a plasma protein)
  • CD14 serves as a receptor for LPS that can play a role in the cellular production of proinflammatory cytokines such as IL-1 and TNF
  • CD14 can be involved in the development of endotoxic shock and LPS-stimulated bone resorption, and promotes, possibly indirectly, bacterial dissemination
  • Flow cytometric analysis demonstrates that rmC5-3 antibody stains J774A.1 (mouse macrophage line), WEHI-265.1 (mouse monocytic line), peritoneal resident macrophages, Kupffer cells, and cultured bone marrow-derived macrophages and dendritic cells, but not unstimulated splenic macrophages, dendritic cells, neutrophils, or blood monocytes
  • This staining pattern is similar to that of the alternate anti-mouse CD14 mAb 4C1/CD14, which recognizes a different CD14 epitope, and differs from that of the human, where CD14 expression is characteristic of circulating monocytes and neutrophils
  • Therefore, data suggests that CD14 expression by leukocyte populations may differ in mice and humans
  • Peritoneal cells from naive mice, 3-day thioglycollate-elicited peritoneal exudate, as well as 4-hour LPS-activated peritoneal cells, contain a population of Mac-1 (CD11b)-high cells which double-stain with rmC5-3 antibody
  • Levels of CD14 expression on Kupffer cells and bone marrow-derived macrophages and dendritic cells of LPS-sensitive mice are increased by in vivo and in vitro LPS treatments, an effect which may be mediated by TNF
  • Preliminary evidence suggests that CD14 may be up-regulated on mouse blood neutrophils
  • In agreement with the observations that CD14 is shed from activated human and mouse monocytes, rmC5-3 mAb detects soluble CD14 in the serum of LPS-treated mice in a time-dependent manner.

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Antigen:
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Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Recombinant Mouse CD14

Quantity:
25 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
rmC5-3

Conjugate:
PE

Host Species:
Rat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

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Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Immunogen:
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Quantity:
25 Tests

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
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Conjugate:
FITC

Host Species:
Mouse

Purification Method:
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Clone:
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Conjugate:
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Host Species:
Mouse

Purification Method:
Affinity Purified

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RUO

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IgG1 κ

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Primary or Secondary:
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Applications:
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Clone:
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Conjugate:
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Host Species:
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Purification Method:
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Regulatory Status:
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Isotype:
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