CD3e Hamster anti-Mouse, FITC, Clone: 145-2C11, BD

Description

• The 145-2C11 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex that is expressed on thymocytes, mature T lymphocytes, and NK-T cells
• The cytoplasmic domain of CD3e participates in the signal transduction events that activate several cellular biochemical pathways as a result of antigen recognition
• Soluble 145-2C11 antibody can activate either unprimed (naive) or primed (memory/preactivated) T cells in vivo or in vitro , in the presence of Fc receptor-bearing accessory cells
• In contrast, plate-bound 145-2C11 can activate T cells in the absence of accessory cells
• Soluble 145-2C11 antibody has been reported to induce re-directed lysis of Fc receptor-bearing target cells by CTL clones and can also block lysis of specific target cells by antigen-specific CTL's
• Under some conditions, T-cell activation by 145-2C11 antibody has been reported to result in apoptotic cell death
• The 145-2C11 antibody does not cross-react with rat leukocytes
• Preincubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining reportedly enhances the ability of anti-CD3ε and anti-αβ TCR mAbs to detect the T-cell receptor on immature thymocytes
• This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.