CD19 Mouse anti-Human, PE-CF594, Clone: HIB19, BD

Description

• The HIB19 monoclonal antibody specifically binds to the 95 kDa type I transmembrane CD19 glycoprotein
• CD19 is expressed during all stages of B-cell maturation and differentiation, except on plasma cells
• CD19 is also present on follicular dendritic cells
• It is not found on T cells or on normal granulocytes
• CD19 is a signal transduction molecule that regulates B cell development, activation, proliferation and differentiation
• It associates with the complement receptor 2 (CD21), TAPA-1 (CD81), Leu 13, and/or MHC class II to form a signal transduction complex on the surface of B cells
• Anti-CD19 clone HIB19 partially blocks the binding of clone B43, another CD19-specific monoclonal antibody
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red (eg 610/20-nm filter).